Effect of peripherally-injected glucagon-like peptide-1 on gastric mucosal blood flow


Isbil-Buyukcoskun N., Cam-Etoz B., Gulec G. , Ozluk K.

REGULATORY PEPTIDES, vol.157, no.1-3, pp.72-75, 2009 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 157 Issue: 1-3
  • Publication Date: 2009
  • Doi Number: 10.1016/j.regpep.2009.04.013
  • Title of Journal : REGULATORY PEPTIDES
  • Page Numbers: pp.72-75
  • Keywords: GLP-1, Ethanol, Gastric mucosal blood flow, L-NAME, CGRP-(8-37), Indomethacin, GENE-RELATED PEPTIDE, EXPERIMENTAL-MODELS, GLP-1, ETHANOL, RAT, SYSTEM, AMIDE, SOMATOSTATIN, INVOLVEMENT, MECHANISMS

Abstract

The aim of this study was to investigate the mechanisms involved in the effect of glucagon-like peptide-1 (GLP-1) on the decrease in gastric mucosal blood flow (GMBF) induced by intragastric ethanol. After preparation of the stomach for GMBF recording, a probe was placed to the gastric mucosa and basal GMBF recordings were obtained by a laser Doppler flowmeter after a 30-minute stabilization period. Following GLP-1 (1000 ng/kg; i.p.) injection, 1 ml of absolute ethanol was applied to the gastric chamber and GMBF was recorded continuously during a 30-minute period. GLP-1 (1000 ng/kg; i.p.) prevented the decrease in GMBF induced by ethanol. Nitric oxide (NO) synthase inhibitor L-NAME, (30 mg/kg; s.c.), calcitonine gene-related peptide (CGRP) receptor antagonist CGRP-(8-37) (10 mu g/kg; i.p.), and cyclooxygenase inhibitor indomethacin (5 mg/kg; i.p.) all inhibited the GMBF-improving effect of GLP-1. We concluded that, NO, CGRP and prostaglandins may be involved in the effect of peripherally-injected GLP-1 on GMBF reduction induced by intraluminal ethanol. Crown Copyright (c) 2009 Published by Elsevier B.V. All rights reserved.