Embryo viability indexing using Raman spectroscopy of spent culture media


Parlatan U., Başar G., Bavili N., Yumru H., Sag F., Bulgurcuoglu S., ...Daha Fazla

SPECTROSCOPY LETTERS, cilt.49, ss.458-463, 2016 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 49 Konu: 7
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1080/00387010.2016.1198814
  • Dergi Adı: SPECTROSCOPY LETTERS
  • Sayfa Sayıları: ss.458-463

Özet

Embryo viability quantification is an important topic for in vitro fertilization researchers. This study suggests Raman spectroscopy as a candidate method to find an objective measure of embryo viability. Raman spectra of 31 spent embryo culture samples (16 nonpregnant, 15 pregnant) from 31 patients were analyzed with band component analysis. The band area ratio of 902 to 943 cm 1 was found to be the most discriminatory. These bands are related to glutamine, glycine, proline, aspartic acid, and valine. According to the pregnancy outcome the sensitivity and specificity of Raman analysis were found to be 93% and 77%, respectively.