Method validation of tandem mass spectrometry for 25-Hydroxyvitamin D3 and comparison of this method with other methods


Sahillioglu B., SERDAR M. A. , Erkal N., Erden G., Bakir F., Yildirimkaya M. M. , ...More

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.36, no.1, pp.73-79, 2011 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 1
  • Publication Date: 2011
  • Title of Journal : TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Page Numbers: pp.73-79

Abstract

Objective: Measurement of circulating 25-hydroxyvitamin D 3 is accepted as the clinical indicator of vitamin D status. It is very important of valid measurement of 25-hydroxyvitamin D 3 as clinical. Therefore there is need a standardization for measurement. Methods except tandem mass spectrometry (LC-MS/MS) measure total vitamin D and are less sensitive. LC-MS/MS is gold standard method for measuring vitamin D values. On this account we have invented a new method and evaluated this method to measure the imprecision and the accuracy and compared it with other systems.