The effects of granulocyte colony-stimulating factor on the heating of tracheal anastomosis following radiation therapy in rats


Kuzucu A., Erkal H. S. , Kirimlioglu H. , BATÇIOĞLU K., Yucel N., Serin M.

EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY, vol.30, no.6, pp.840-845, 2006 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 6
  • Publication Date: 2006
  • Doi Number: 10.1016/j.ejcts.2006.09.023
  • Title of Journal : EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
  • Page Numbers: pp.840-845

Abstract

Objective: This study evaluates the effects of granulocyte colony-stimulating factor on the heating of tracheal anastomosis following radiation therapy in rats. Methods: Fifty-six mate Wistar rats were divided into four groups. Group 1 underwent tracheal anastomosis. Group 2 underwent radiation therapy followed by tracheal anastomosis. Group 3 underwent radiation therapy followed by tracheal. anastomosis and received granulocyte colony-stimulating factor. Group 4 underwent sham radiation therapy followed by sham tracheal. anastomosis. At 10 days following radiation therapy, the trachea was dissected for histopathological, mechanical and biochemical evaluation. Results: Median scores for inflammation were three points for Group 1, two points for Group 2, two points for Group 3 and one point for Group 4. Median scores for angiogenesis were four points for Group 1, two points for Group 2, three points for Group 3 and one point for Group 4. Median scores for connective tissue regeneration were four points for Group 1, two points for Group 2, three points for Group 3 and one point for Group 4. Median scores for epithelial regeneration were two points for Group 1, one point for Group 2, one point for Group 3 and one point for Group 4. Mean anastomotic bursting pressures were 853 mmHg for Group 1, 293 mmHg for Group 2, 417 mmHg for Group 3 and 966 mmHg for Group 4. Mean hydroxyproline concentrations were 159 mu g/mg for Group 1, 177 mu g/mg for Group 2, 120 mu g/mg for Group 3 and 117 mu g/mg for Group 4. Conclusions: This study suggests that granulocyte colony-stimulating factor contributes to the heating of tracheal anastomosis following radiation therapy through improved connective tissue regeneration. (c) 2006 Elsevier B.V. All rights reserved.