Molecular characterization of cDNA encoding resistance gene-like sequences in Buchloe dactyloides

Budak H., Kasap Z., Shearman R. C., Dweikat I., Sezerman U., Mahmood A.

MOLECULAR BIOTECHNOLOGY, cilt.34, sa.3, ss.293-301, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 3
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1385/mb:34:3:293
  • Dergi Adı: MOLECULAR BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.293-301
  • Anahtar Kelimeler: buffalograss, cDNA, molecular evolution, resistance genes, RACE, NUCLEOTIDE-BINDING SITE, DOWNY MILDEW RESISTANCE, DISEASE-RESISTANCE, RUST RESISTANCE, ATP-BINDING, LOCUS, EVOLUTION, ANALOGS, RICE, BACTERIAL
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

Current knowledge of resistance (R) genes and their use for genetic improvement in buffalograss (Buchloe dactyloides [Nutt.] Engelm.) lag behind most crop plants. This study was conducted to clone and characterize cDNA encoding R gene-like (RGL) sequences in buffalograss. This report is the first to clone and-characterize of buffalograss RGLs. Degenerate primers designed from the conserved motifs of known R genes were used to amplify RGLs and fragments of expected size were isolated and cloned. Sequence analysis of cDNA clones and analysis of putative translation products revealed that most encoded amino acid sequences shared the similar conserved motifs found in the cloned plant disease resistance genes RPS2, MLA6, L6, RPM1, and Xa1. These results indicated diversity of the R gene candidate sequences in buffalograss. Analysis of 5' rapid amplification of cDNA ends (RACE), applied to investigate upstream of RGLs, indicated that regulatory sequences such as TATA box were conserved among the RGLs identified. The cloned RGL in this study will further enhance our knowledge on organization, function, and evolution of R gene family in buffalograss. With the sequences of the primers and sizes of the markers provided, these RGL markers are readily available for use in a genomics-assisted selection in buffalograss.