A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening


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Chen Y., Scanlan M., Sahin U., Tureci O., Gure A. O. , Tsang S., ...More

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol.94, no.5, pp.1914-1918, 1997 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 94 Issue: 5
  • Publication Date: 1997
  • Doi Number: 10.1073/pnas.94.5.1914
  • Title of Journal : PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Page Numbers: pp.1914-1918

Abstract

Serological analysis of recombinant cDNA expression libraries (SEREX) using tumor mRNA and autologous patient serum provides a powerful approach to identify immunogenic tumor antigens, We have applied this methodology to a case of esophageal squamous cell carcinoma and identified several candidate tumor targets, One of these, NY-ESO-1, showed restricted mRNA expression in normal tissues, with high-level mRNA expression found only in testis and ovary tissues, Reverse transcription-PCR analysis showed NY-ESO-I mRNA expression in a variable proportion of a wide array of human cancers, including melanoma, breast cancer, bladder cancer, prostate cancer, and hepatocellular carcinoma, NY ESO-1 encodes a putative protein of Mr 17,995 having no homology with any known protein, The pattern of NY-ESO-1 expression indicates that it belongs to an expanding family of immunogenic testicular antigens that are aberrantly expressed in human cancers in a lineage-nonspecific fashion, These antigens, initially detected by either cytotoxic T cells (MAGE, BAGE, GAGE-1) or antibodies [HOM-MEL-40(SSX2), NY-ESO-1], represent a pool of antigenic targets for cancer vaccination.