A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening


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Chen Y., Scanlan M., Sahin U., Tureci O., Gure A. O. , Tsang S., ...Daha Fazla

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, cilt.94, sa.5, ss.1914-1918, 1997 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 94 Konu: 5
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1073/pnas.94.5.1914
  • Dergi Adı: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Sayfa Sayıları: ss.1914-1918

Özet

Serological analysis of recombinant cDNA expression libraries (SEREX) using tumor mRNA and autologous patient serum provides a powerful approach to identify immunogenic tumor antigens, We have applied this methodology to a case of esophageal squamous cell carcinoma and identified several candidate tumor targets, One of these, NY-ESO-1, showed restricted mRNA expression in normal tissues, with high-level mRNA expression found only in testis and ovary tissues, Reverse transcription-PCR analysis showed NY-ESO-I mRNA expression in a variable proportion of a wide array of human cancers, including melanoma, breast cancer, bladder cancer, prostate cancer, and hepatocellular carcinoma, NY ESO-1 encodes a putative protein of Mr 17,995 having no homology with any known protein, The pattern of NY-ESO-1 expression indicates that it belongs to an expanding family of immunogenic testicular antigens that are aberrantly expressed in human cancers in a lineage-nonspecific fashion, These antigens, initially detected by either cytotoxic T cells (MAGE, BAGE, GAGE-1) or antibodies [HOM-MEL-40(SSX2), NY-ESO-1], represent a pool of antigenic targets for cancer vaccination.