ABL gene kinase domain mutation scanning by denaturing high performance liquid chromatography sequencing method Denatüre edici yüksek performanslı likit kromotografi yöntemi ile ABL geni kinaz bölgesi mutasyonlarının taranması


Erbilgin Y., Catal S., Eskazan A. E. , Hatirnaz Ö. , Soysal T., Ozbek U.

Turkish Journal of Hematology, vol.28, no.2, pp.97-102, 2011 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 2
  • Publication Date: 2011
  • Doi Number: 10.5152/tjh.2011.24
  • Title of Journal : Turkish Journal of Hematology
  • Page Numbers: pp.97-102

Abstract

Objective: Despite the efficacy of the BCR-ABL tyrosine kinase inhibitor imatinib, the development of resistance against imatinib has been observed. The most important mechanisms known to cause resistance are point mutations in the ABL tyrosine kinase and the ATP domain. This study describes the use of denaturing high performance liquid chromatography (dHPLC) as a method to screen for mutations of the ABL gene.