Background: Ankaferd Blood Stopper (ABS) is a plant extract and it has been used as a haemostatic agent in Turkish traditional medicine. ABS could be used in intraperitoneal bleeding due to any surgery or trauma. However, it is not clear, whether it has any side effect in peritoneum such as adhesions. In our study, we aimed to evaluate the ABS as a possible source of intraperitoneal adhesions. Materials and methods: The study was conducted with thirty Wistar albino female rats. The rats were divided into three equal groups. Group C was control group with laparotomy only. Group S was Surgicel(R) (regenerated oxidized cellulose) group with laparotomy and bleeding on the uterus and application of Surgicel (1cmx1cm) to the bleeding site. Group A was the ABS group constituted in uterus bleeding and application of ABS to the site of injury (2 ml). After 21 days, all rats were killed. We scored the adhesions according to their degree of severity and collected specimen for the detection of hydroxyproline levels. Results: Macroscopically, there was grade 1 and 2 adhesions in ABS group (median score: 1). In control and Surgicel group, there was no significant adhesion (median score: 0). The mean hydroxyproline level of the control group was significantly higher than Surgical and ABS groups (3.28, 2.11, and 2.48 mg/g wet tissue respectively). Conclusion: Application of ABS for intraperitoneal bleeding may cause intraperitoneal adhesion. The relatively low levels of hydroxproline in ABS group led us to think that ABS causes adhesions by a mechanism that not due to newly organized collagen. Although ABS is a useful haemostatic substance, care must be taken during its intraperitoneal application.