Analysis of a mini-outbreak of methicillin-resistant Staphylococcus aureus in a surgical ward by using arbitrarily primed-polymerase chain reaction

Cetinkaya Y., Kocagoz S., Hayran M., Uzun O., Akova M., Gursu G., ...Daha Fazla

JOURNAL OF CHEMOTHERAPY, cilt.12, sa.2, ss.138-144, 2000 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 12 Sayı: 2
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1179/joc.2000.12.2.138
  • Dergi Adı: JOURNAL OF CHEMOTHERAPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.138-144
  • Anahtar Kelimeler: methicillin-resistant Staphylococcus aureus, nosocomial outbreaks, arbitrarily primed polymerase chain reaction, PCR, POLYMORPHIC DNA ASSAY, STRAINS, INFECTION, CARRIAGE, EPIDEMIOLOGY, COLONIZATION, EVOLUTION, SPREAD, PCR
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

resistant Staphylococcus aureus (MRSA) isolates from a surgical ward at Hacettepe University Hospital. All MRSA isolates obtained from clinical specimens in this ward (14 MRSA isolates from wound cultures of 10 patients) were collected prospectively for 10 weeks. Surveillance cultures were taken from ward personnel (nose cultures from 4 physicians, 7 nurses, 1 secretary, 1 waiter), 2 surgical dressing containers and 1 nebulizer, MRSA was isolated from one of the surgical dressing containers, the nebulizer and nose cultures of 3 physicians, 3 nurses and the ward secretary. Arbitrarily primed polymerase chain reaction (AP-PCR) analysis showed that most MRSA isolates belonged to 2 major clones (pattern A, pattern B), Pattern A was the most frequent one and was present in 4 clinical isolates, surgical dressing container-1. Pattern B was identified in 3 clinical isolates and nose culture of physician-3, AP-PCR analysis revealed that this mini-MRSA outbreak was caused by contamination of surgical dressing container with MRSA and nasal MRSA carriage in ward staff. AP-PCR seems to be a valuable typing method for analysis of nosocomial MRSA outbreaks because of ifs simplicity and rapidity.