Akademik Veri Yönetim Sistemi
HIBIT2024 (17th International HIBIT Conference), İstanbul, Türkiye, 18 - 20 Aralık 2024, ss.109, (Özet Bildiri)
Background: Obesity is a preventable disease resulting from genetic, environmental, and lifestyle factors. While microbiota has been implicated as a contributor to obesity, most studies have focused on fecal samples, potentially overlooking the small intestine's role in food digestion and nutrient absorption. Additionally, these studies predominantly focus on bacteria, with fungi receiving less attention in the context of obesity. This study aims to examine the diÉerences in bacterial and fungal microbiota composition in the duodenal tissue of obese and normal-weight individuals using metagenomics. Methods: Five obese patients (BMI >35 kg/m²) and five normal-weight control patients (BMI <25 kg/m²) were included in this study. After (DNA) extraction from duodenal tissue samples, 16S rRNA gene sequencing was performed to identify bacteria, and ITS sequencing was used for fungi. Bioinformatic analysis was conducted using QIIME2 to assign taxonomic classifications to the bacterial and fungal sequences. Results: In bacterial communities, the most abundant phyla were Firmicutes, Proteobacteria, Bacteroidetes, and Actinobacteria. Streptococcus was the most common at the genus level, followed by Lactobacillus and Prevotella. For fungi, two phyla were identified: Ascomycota and Basidiomycota. Candida was more prevalent at the genus level, with species such as Candida glabrata, Candida dubliniensis, and Candida tropicalis observed exclusively in the control group. In contrast, Malassezia was observed only in obese patients. Conclusion: This study revealed diÉerences in the duodenal bacterial and fungal microbiota composition between obese and normal-weight individuals, highlighting the potential role of duodenal microbiota in obesity. These findings suggest that specific microbial taxa may be associated with obesity. Future studies with larger sample sizes and diverse populations are needed further to elucidate the relationship between obesity and gut microbiota.