Heme-regulated eIF-2 alpha kinase purifies as a hemoprotein

Chefalo P., Oh J., Rafie-Kolpin M., Kan B., Chen J.

EUROPEAN JOURNAL OF BIOCHEMISTRY, cilt.258, sa.2, ss.820-830, 1998 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 258 Sayı: 2
  • Basım Tarihi: 1998
  • Doi Numarası: 10.1046/j.1432-1327.1998.2580820.x
  • Dergi Adı: EUROPEAN JOURNAL OF BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.820-830
  • Anahtar Kelimeler: protein kinase, heme, hemoprotein, translation, initiation, INITIATION FACTOR-II, GCN2 PROTEIN-KINASE, HEAT-SHOCK-PROTEIN, T-CELL ACTIVATION, MALIGNANT TRANSFORMATION, ALPHA-SUBUNIT, RETICULOCYTE LYSATE, NITRIC-OXIDE, SPECTRAL CHARACTERIZATION, TRANSLATIONAL INHIBITOR
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

The regulation of protein synthesis by the availability of heme in reticulocytes is well established. However, the mechanism by which heme regulates translational initiation is not clear. In this study, we have examined the heme regulation directly on the homogeneous heme-regulated eIF-2 alpha kinase (HRI), which is activated during heme deficiency. We found that HRI purified as a hemoprotein with the characteristic Soret band of hemoprotein at 424 nm. This HRI was an active autokinase and eIF-2 alpha kinase, and its kinase activities were inhibited by submicromolar concentrations of hemin with an apparent K, of 0.5 mu M Homogeneous HRI was a homodimer, and its activities could not be inhibited by incubation with purified inactive K199R HRI in vitro. Our results suggest that there are two distinct types of heme-binding sires in the HRI homodimer. The binding of heme to the first site is stable, while the binding of heme to the second site is responsible for the rapid downregulation of HRI activity by heme, These results indicate that HRI binds heme and sen es as a sensor of the availability of heme to coordinate the balanced synthesis of globins and heme in erythroid cells.