Akademik Veri Yönetim Sistemi
IV Bioactive Natural Products Research Conference (IV. Bio.Natural), Lisbon, Portekiz, 14 - 15 Temmuz 2025, ss.1, (Özet Bildiri)
Tribulus terrestris L. is an annual flowering plant from Zygophyllaceae family, which has been used in traditional medicine for ages.[1] Studied therapeutic activities of T. terrestris include anti-urolithic, cardioprotective, anti-inflammatory, anti-diabetic, hypolipidemic, anti-cancer, neuro-protective, testosterone-boosting and many more. Saponins and flavonoids are the chief phytochemicals that were found in T. terrestris plant, as well as being majorly responsible for the therapeutic effects.[2] Despite ongoing research on its bioactivities, there is no assessment of human digestion system on phenolic composition and antioxidant activity of T. terrestris. Studies indicate that the human digestive system has a significant impact on the bioavailability of herbal medications.[3] Therefore, in this study, phenolic profile and antioxidant activity of T. terrestris methanolic extracts before and after the in vitro gastrointestinal human digestion simulation were measured and each 3 fraction were compared to one another (BFR: non-digested/before, PG: post-gastric, IN: serum-available/bioavailable). After the simulation; metal reducing antioxidant activities (CUPRAC and FRAP), DPPH radical scavenging activity, total antioxidant content (ToAC) assays were performed for each fraction of the digestion simulation. In addition, phenolic profile was clarified with total flavonoid content (TFC), total phenolic content (TPC) and high performance thin-layer chromatography (HPTLC). Rutin was detected and quantitative analysis was performed. Assays showed that antioxidant activities and phenolic analyses of T. terrestris extract were decreased during digestion simulation. The obtained data illustrate the significance of digestion regarding the bioavailability of ingested herbal products.