Akademik Veri Yönetim Sistemi
ULUSAL TRAVMA VE ACIL CERRAHI DERGISI-TURKISH JOURNAL OF TRAUMA & EMERGENCY SURGERY, cilt.31, sa.2, ss.95-102, 2025 (SCI-Expanded, Scopus)
BACKGROUND: Infertility and organ loss are potential consequences of testicular torsion, a urological emergency. This study aimed to evaluate the impact of lupeol on testicular ischemia-reper fusion damage. METHODS: Thirty adult male Sprague-Dawley rats were randomly assigned to five groups: Control (C), Lupeol (L), Ischemia (Isc), Treatment 1 (T1), and Treatment 2 (T2). In the study groups, detorsion was applied to the left testicles following the induction of 720-degree testicular torsion for two hours. In the T1 and T2 groups, 100 mg/kg of lupeol was administered intraperitoneally 30 minutes before and immediately after detorsion. At the sixth hour, blood and testicular tissue samples were collected from each rat. Measurements included serum interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), tissue glutathione (GSH), malondialdehyde (MDA), and caspase-3 levels. Histopathological analysis was performed to assess the Johnsen Tubular Biopsy Score (JTBS). RESULTS: Levels of caspase-3 (2.74 +/- 0.32), MDA (1.71 +/- 0.26), IL-6 (4.92 +/- 0.57), and TNF-alpha (113.18 +/- 29.77) were elevated in Group Isc compared to Group C and showed a significant reduction in Group T2 (2 +/- 0.67, 1.16 +/- 0.36, 3.95 +/- 0.17, and 106.13 +/- 12.49, respectively) and particularly in Group T1 (1.65 +/- 0.50, 0.95 +/- 0.143, 80 +/- 0.35, and 104.86 +/- 8.42, respectively) (p=0.001). However, while TNF-alpha levels decreased in both treatment groups, the difference was not statistically significant (p=0.768). GSH levels decreased in Group Isc (140.63 +/- 25.71) but increased in Group T2 (211.58 +/- 95.05) (p=0.753) and particularly in Group T1 (219.9 +/- 48.21) (p=0.078). The JTBS was lowest in Group Isc (7.67 +/- 0.25). However, improvements were observed in both treatment groups (8.93 +/- 0.16 and 8.82 +/- 0.22, respectively) (p=0.001). CONCLUSION: This study, the first to use lupeol in an experimental testicular torsion model, demonstrated its antioxidant, antiinflammatory, anti-apoptotic, histopathological damage-reducing, and protective effects.