Serum prolidase and IGF-1 as non-invasive markers of hepatic fibrosis during four different periods after bile-duct ligation in rats

Tarcin O., Gedik N., Karakoyun B., Tahan V., Sood G., ÇELİKEL Ç., ...Daha Fazla

DIGESTIVE DISEASES AND SCIENCES, cilt.53, sa.7, ss.1938-1945, 2008 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 7
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1007/s10620-007-0073-1
  • Dergi Adı: DIGESTIVE DISEASES AND SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1938-1945
  • Anahtar Kelimeler: prolidase, IGF-1, collagen, hepatic fibrosis, serum marker, bile-duct ligation, GROWTH-FACTOR-I, LIVER FIBROSIS, PLASMA PROLIDASE, BINDING-PROTEIN, CIRRHOSIS, COLLAGEN, INDEX, HORMONE, DISEASE, PROGRESSION
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Evet

Özet

Aim Our aim was to study the correlation of serum prolidase and insulin like growth factor-1 to liver collagen and assess their utility as markers of fibrosis during four different periods of hepatic injury and fibrosis after bile-duct ligation in rats. Methods Forty-eight Wistar albino rats were included in the study and divided into six groups. Seven rats served as the control group (Control), while seven rats had a sham operation (Sham group). Thirty-four rats underwent bile-duct ligation. Bile-duct ligated (BDL) animals were sacrificed at the end of the first week (Group 1; n = 8), second week (Group 2; n = 8), third week (Group 3; n = 9), or fourth week (Group 4; n = 9) after BDL. Liver collagen, liver prolidase, and serum prolidase and IGF-1, were determined. Results There was a positive correlation between liver collagen and serum prolidase (r(s): 0.843, P < 0.001) levels and a negative correlation among liver collagen and serum IGF-1 levels (r(s): -0.667, P < 0.001). The peak levels of liver collagen and serum prolidase were reached in the third week while the lowest levels of IGF-1 were found at the end of the third week. Conclusion Serum prolidase and IGF-1 either independently or in combination correlate with liver collagen content in hepatic fibrosis.