Introduction: We aim to investigate Central venous catheters (CVC)-related blood stream infection (CVCR-BSI) rate for CVCR-BSI in dialysis patients with acute or chronic kidney disease at our unit. Method: A total of 67 inpatients with kidney failure who underwent CVC insertion between May 2019 and May 2020 participated in this prospective, single-center, observational study. Patients were divided into two groups. Patients without CVCR-BSI were recruited into group 1, and those with CVCRBSI were recruited into group 2. The demographics, clinical and laboratory variables, duration of hospital stay, causative pathogen, type and duration of treatment, and outcome of the infection of the groups were recorded and compared. Results: CVCR-BSI was not diagnosed in 52 patients (group 1) and 15 patients were diagnosed with CVCR-BSI (group 2). The groups were similar. The major isolated microorganisms were Staphylococcus aureus and epidermidis. The average time of CVCR-BSI development was 10.2 ± 8.2 days after insertion of CVC. The mean time to detection of microorganism in the blood culture was 12.3 ± 7.4 hours, while the time to positivity of blood culture drawn through the CVC hub was 16.5 ± 6 hours. C-reactive protein levels of group 2 were significantly higher at the time of infection compared to baseline levels (p<0.05). Conclusion: We found that CVCR-BSI developed approximately 10 days after CVC insertion. Time to culture positivity was documented for nearly 12 hours. Elevation in CRP levels was found to be a significant marker for suspicion of CVCR-BSI.