Akademik Veri Yönetim Sistemi
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, cilt.236, ss.183-192, 2019 (SCI-Expanded)
Objective: To investigate the paternal mitochondrial DNA's effect on assisted reproductive technology (ART) applications and possible paternal mitochondrial DNA transmission in male factor infertility diagnosed fathers. Study design: Study group was designed according to the families all of which applied to assisted reproductive technologies as a result of male infertility. A total of 16 trios (48 mother-father-child samples) which contain 7 newborns and 9 infants born by in vitro fertilization method (IVF-ICSI) were studied using "Illumina, MiSeq" next-generation sequencing platform (Case-parent trio study). The study has been conducted between February 2017 and May 2018. Result(s): Sequencing analysis results were investigated on the basis of "mother-father-child", "mother-child" and "father-child" mitochondrial DNA whole genome sequence data, respectively. In 14 "trios" of 16; maternal mitochondria] DNA haplotype were detected for children, the remaining 2 "trios" had different mitochondrial DNA haplotypes when compared to their mother and fathers. Also; "father-child" sharing same genetic variants (SNP ("Single nucleotide polymorphism") / MNP ("Multiple nucleotide polymorphism") / INDEL ("Insertion/Deletion") were found in 8 "trios". In 5 "trios" of 16; 98-99% paternal mitochondrial DNA genome sequence similarity were obtained by alignment of "father-child" mitochondria] DNA genome. Conclusion(s): This study is the first whole mitochondrial genome investigation for paternal mitochondrial DNA contribution in human IVF / ICSI applied trio cases. Our findings for paternally derived variants could be the result of intermolecular recombination between maternal and paternal mitochondrial DNA. (C) 2019 Elsevier B.V. All rights reserved.