Retinal pigment epithelium cell culture on surface modified poly(hydroxybutyrate-co-hydroxyvalerate) thin films


TEZCANER A., Bugra K., Hasirci V. N.

BIOMATERIALS, vol.24, no.25, pp.4573-4583, 2003 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 25
  • Publication Date: 2003
  • Doi Number: 10.1016/s0142-9612(03)00302-8
  • Journal Name: BIOMATERIALS
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.4573-4583
  • Keywords: retinal pigment epithelium, cell culture, tissue engineering, PHBV, biodegradable, ANTIBIOTIC RELEASE, TISSUE, TRANSPLANTATION, ADHESION, DEGENERATION, POLY(3-HYDROXYBUTYRATE), BIOMATERIALS, FIBRONECTIN, DEGRADATION, ROUGHNESS

Abstract

There is currently no effective treatment for the retinal disorders caused by retinal pigment epithelium (RPE) degeneration. Transplantation of allografts is the main strategy towards correction of this malady. Tissue engineering could offer hope and involve the use of biodegradable polymeric templates to replace diseased or lost RPE. In this study PHBV8 film was chosen as a temporary substrate for growing retinal pigment epithelium cells as an organized monolayer before their subretinal transplantation. The surface of the PHBV8 film was rendered hydrophilic by oxygen plasma treatment to increase the reattachment of D407 cells on the film surface.