Synthetic antibodies for methamphetamine analysis: Design of high affinity aptamers and their use in electrochemical biosensors
Journal of Electroanalytical Chemistry, cilt.921, 2022 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 921
- Basım Tarihi: 2022
- Doi Numarası: 10.1016/j.jelechem.2022.116686
- Dergi Adı: Journal of Electroanalytical Chemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
- Anahtar Kelimeler: Abused drugs, Aptasensors, Biosensors, Electrochemical biosensors, Methamphetamine
- Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Evet
Özet
An aptamer-based electrochemical biosensor was designed and fabricated for methamphetamine (METH) analysis. Several aptamer sequences developed by the GO-SELEX method were immobilized onto gold electrodes (GE) via gold-thiol affinity. Surface characterizations were performed by electrochemical methods including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectrometry (EIS). GE/Apta-4/METH aptasensor was selected for further studies since the best results were obtained with the Apta-4 sequence in terms of analytical parameters. Accordingly, it exhibited good selectivity for METH analysis with a wide linear range of 0.1–50 ng/mL and a low limit of detection (LOD) which was found to be 0.467 ng/mL. The proposed aptasensor platform displayed good selectivity for the detection of METH in synthetic urine samples spiked with METH.