Synthetic antibodies for methamphetamine analysis: Design of high affinity aptamers and their use in electrochemical biosensors

Bor G., Bulut U., Man E., Balaban Hanoglu S., Evran S., Timur S.

Journal of Electroanalytical Chemistry, vol.921, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 921
  • Publication Date: 2022
  • Doi Number: 10.1016/j.jelechem.2022.116686
  • Journal Name: Journal of Electroanalytical Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
  • Keywords: Abused drugs, Aptasensors, Biosensors, Electrochemical biosensors, Methamphetamine
  • Acibadem Mehmet Ali Aydinlar University Affiliated: Yes

Abstract

An aptamer-based electrochemical biosensor was designed and fabricated for methamphetamine (METH) analysis. Several aptamer sequences developed by the GO-SELEX method were immobilized onto gold electrodes (GE) via gold-thiol affinity. Surface characterizations were performed by electrochemical methods including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectrometry (EIS). GE/Apta-4/METH aptasensor was selected for further studies since the best results were obtained with the Apta-4 sequence in terms of analytical parameters. Accordingly, it exhibited good selectivity for METH analysis with a wide linear range of 0.1–50 ng/mL and a low limit of detection (LOD) which was found to be 0.467 ng/mL. The proposed aptasensor platform displayed good selectivity for the detection of METH in synthetic urine samples spiked with METH.