Research Information System
15th European Epilepsy Congress, Rome, Italy, 7 - 11 September 2024, vol.65, pp.970-971, (Full Text)
PURPOSE:
Temporal lobe epilepsy (TLE) is a neurological disease characterized by recurrent seizures originating from the temporal lobes of the brain. These seizures can manifest as cognitive disturbances, perception alterations, and sometimes loss of consciousness and convulsions (Tatum IV et al. J Clin Neurophysiol 2012;29(5):356-365). We know that 60-70% of patients with epilepsy can achieve seizure freedom with the appropriate use of anti-seizure drugs (ASDs) yet nearly one-third of them do not respond to ASDs (Löscher W et al. Pharmacol Rev. 2020;72(3):606-638). The kindling model is a well-accepted animal model representing TLE (Goddard G et al. Exp Neurol. 1969;25(3):295-330). Here, we aimed to investigate the possible therapeutic effect of 100 mg/kg levetiracetam (LEV) on TLE using the kindling model.
METHOD:
Four cortical recording and one stimulating electrodes were implanted into the basolateral amygdala of male Wistar rats. After 1-week recovery, the kindling started at their after-discharge (AD) threshold for each animal and continued until animals had 3 consecutive stage 5 seizures according to Racine’s scale (Racine RJ et al. Electroencephalogr Clin Neurophysiol. 1972;32(3):281-294) and they were counted as “kindled”. Then, the animals were grouped into LEV (n=7) and control (n=7) groups. LEV group was treated with 100 mg/kg LEV intraperitoneally (i.p.) and the control group was treated with saline i.p. one hour before stimulations. Data were collected as Racine’s stages, AD and total seizure durations from EEG. Then, data were statistically analyzed with 2-way ANOVA test via Graphpad Prism.
RESULTS:
The Racine’s stages (p<0.0001) were significantly reduced in the LEV group compared to the control group.
The amygdala AD durations (p<0.005) were significantly reduced in the LEV group compared to the control group.
Total seizure durations (p<0.005) were significantly reduced in the LEV group compared to the control group.
CONCLUSION:
Our data have shown that LEV treatment has a therapeutic effect on TLE with a 100 mg/kg dose. Upcoming studies are crucial for ontogenic studies and also to test various doses for the mentioned effect.