Akademik Veri Yönetim Sistemi
XXVII SCHOOL OF PURE AND APPLIED BIOPHYSICS Extracellular vesicles: from biophysical to translational challenges, Venice, İtalya, 6 - 10 Şubat 2023, ss.16, (Özet Bildiri)
Quantum dots are semiconductor inorganic nanocrystals that can radiate well thanks to their high quantum efficiency. They are preferred as medical imaging agents in terms of chemical and light sensitivity, since, they are more stable than fluorophores and have high absorption coefficient. Carbon quantum dots have high potential as bioimaging agents in the diagnosis of certain diseases such as cancer, and are more preferred in biological integrations due to their good and long-term luminescence properties and low cytotoxicity. Hot injection method is a rapid method to synthesize not just only carbon quantum dots but also any quantum dots. Here, we report the synthesis of carbon quantum dots from 98% sulfuric acid (H2SO4) and an oxygen-containing organic solvent, acetone, with hot injection method. The synthesis was done by heating 5 mL of 98% H2SO4 with 2.5 mL acetone (volume ratio 2:1) at 170°C in approximately 1 minute and the mixture was neutralized with 5 M sodium hydroxide (NaOH). The synthesized carbon quantum dots were purified by centrifugation for 3 times at 6,000 rpm for 15 minutes. The purified carbon quantum dots’ characterization was done with UV-Vis Spectrophotometer, Fourier-Transform Infrared Spectroscopy (FT-IR), Energy Dispersive Spectroscopy (EDS), and Zeta Potential analysis, Transmission Electron Microscope (TEM), Scanning Electron Microscope (SEM), and fluorescent microscope. The UV-Vis analysis showed that the carbon quantum dots gave absorbance peak at 287 nm. In EDS analysis, only carbon and oxygen elements were observed which indicated that the carbon quantum dots were purified. Fluorescence imaging was done using 10x, 20x, and 40x objective lenses in Zeiss Axiovert inverted fluorescent microscope. Zeta Potential analysis revealed that carbon quantum dots were negatively charged. The TEM images showed that the samples’ diameter was approximately 50 nm, which indicated that there might be aggregation, therefore, the synthesis will be repeated and optimization of this method will be done. These synthesized and characterized carbon quantum dots will be used as bioimaging agents for the designation of cancer cells in a further study.