In Vitro Anti-Aging Potential Evaluation of Maclura pomifera (Rafin.) Schneider 80% Methanol Extract with Quantitative HPTLC Analysis


BARAK T. H. , KURT CELEP İ., AVCI T. B. , BARDAKCI H., CELEP M. E.

Turkish Journal of Pharmaceutical Sciences, vol.19, no.4, pp.400-407, 2022 (ESCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 4
  • Publication Date: 2022
  • Doi Number: 10.4274/tjps.galenos.2021.65087
  • Journal Name: Turkish Journal of Pharmaceutical Sciences
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier, CAB Abstracts, EMBASE, International Pharmaceutical Abstracts, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.400-407
  • Keywords: Maclura pomifera, anti-aging, antioxidants, HPTLC, osajin, OSAGE ORANGE, NEUTROPHIL ELASTASE, HUMAN SKIN, ANTIOXIDANT, ISOFLAVONES, MITOCHONDRIA, PROFILE, OSAJIN, FRUITS, PLANTS
  • Acibadem Mehmet Ali Aydinlar University Affiliated: Yes

Abstract

© 2022, Turkish Pharmacists Association. All rights reserved.Objectives: Maclura pomifera (Rafin.) Schneider is a widespread species all around the world, which is also frequently cultured for ornamental purposes. Previous studies revealed that M. pomifera fruits are rich in prenylated isoflavonoids, exhibit noteworthy biological activities, and have probable benefits, particularly, when applied topically. Considering that phenolic compounds are important sources in the development of antiaging cosmetic products, this study investigated the anti-aging potential of M. pomifera 80% methanolic extract (MPM) by evaluating antioxidant and extracellular matrix degrading enzymes inhibiting activity. Materials and Methods: For this study, the inhibitory potential of 80% MPM against different enzymes associated with the aging process was evaluated. Given the unequivocal role of oxidative stress in aging, in vitro antioxidant tests were employed as well. Moreover, osajin was determined as the major bioactive isoflavonoid of the sample by high performance thin layer chromatography analysis. Results: Results of the mechanistically different antioxidant assays exhibited notable antioxidant potential of the extract. Inhibition potential of MPM against hyaluronidase, collagenase, and elastase enzymes, which are directly linked to acceleration of the aging process, was investigated and results revealed that MPM inhibited the aforementioned enzymes explicitly. MPM had notable phenolic and flavonoid content; 113.92 ± 2.26 mg gallic acid equivalent/g and 66.41 ± 0.74 mg QE/g, respectively. When total antioxidant capacity assays were considered, it is possible to suggest that MPM is a promising anti-aging agent. Conclusion: As a result, this study discloses that extracts of fruits of M. pomifera have significant anti-aging potential and may be used for this purpose.