Dientamoeba fragilis is a flagellated protozoon dwelling in the colonic lumen. It was shown that it may cause clinical manifestations which required effective anti-microbial therapy. General features of 100 D. fragilis-infected patients were assessed in a study conducted in the Parasitology Laboratory of Celal Bayar University Hospital. Patients were mostly females, between 18 and 50 years, and almost two-thirds of them were admitted with gastrointestinal complaints such as bloating, abdominal pain, diarrhea and fatigue. Thirty-four of 100 patients were coinfected with Blastocystis sp., while 27 of 83 (32.5%) patients that submitted three cellophane tape samples were coinfected with Enterobius vermicularis. Another significant outcome of the study was that 7 and 6 of 35 patients reported dermatologic complaints as itching and lesions, consecutively. The patients and/or their families were mostly educated, middle-class individuals living in apartments in city centre, using tap water for drinking and do regular hand-washing. The study results suggest that D. fragilis should be regarded as a causative agent in pediatric and adult patients suffering from abdominal pain, diarrhea and intense bloating, and the fresh stool samples should be examined directly or kept in a fixative solution until examination with a permanent-stained smear such as trichrome.