Sequence specific recognition of ssDNA by fluorophore 3-hydroxyflavone

Capan A., BOSTAN M. H., Mozioglu E., Akoz M., Goren A. C., EROĞLU M. S., ...More

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, vol.153, pp.391-396, 2015 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 153
  • Publication Date: 2015
  • Doi Number: 10.1016/j.jphotobiol.2015.10.019
  • Journal Name: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.391-396
  • Keywords: Fluorophore, 3-Hydroxyflavone, DNA, Specific recognition, Spectroscopic methods, PROTON-TRANSFER, FLUORESCENCE, PROBES, ENVIRONMENT
  • Acibadem Mehmet Ali Aydinlar University Affiliated: Yes

Abstract

A fully water soluble 3-hydroxyflavone (3HF) derivative, N-(3-hydroxy-4'-flavonyl)-N,N,N-trimethylammonium sulfate (3HFNMe3) was synthesized. Investigation of its emissions at varying wavelengths revealed that it had three emission bands of normal (N*), anionic (A*) and tautomeric (T*), in ultrapure water. Recognition of single-stranded ten ssDNA chains, having different nucleotide sequences was studied, using the ratiometric change of the intensities of the two bands (A*/T*), depending upon the varying environment of the 3HFNMe3 with different ssDNA chains. Addition of the ssDNA chains to the 3HFNMe3 solution caused gradual quenching of the A* band and had almost no effect on the T*. band. As the ratios of the two bands (A*/T*) vs increasing amount of the ssDNAs generated characteristic curves for each ssDNA chain, it became possible to identify the chains with their characteristic curves. (C) 2015 Elsevier B.V. All rights reserved.