The objective of the present study was to describe some reproductive parameters, hormonal levels and some biochemical properties of blood serum in rams under the Afyonkarahisar province conditions. Semen (via an artificial vagina) and blood were collected five times during the breeding season from a total of 10 mature and healthy fat-tailed rams (Akkaraman and Awassi) in Afyonkarahisar. Breed effects were significant for relative testes volume, sperm concentration, percentage of abnormal spermatozoa, triioclothyronine (T-3), albumin/globulin ratio, total lipid (P < 0.05) and cholesterol (P < 0.01). Some reproductive parameters and biochemical properties of Akkaraman and Ivesi rams indicated that the values were recorded in the optimum range during the breeding season under Afyonkarahisar conditions. In addition, some reproductive parameters of rams show a correlation with some biochemical and enzymatic properties.