Akademik Veri Yönetim Sistemi
ACTA BOTANICA BRASILICA, cilt.39, 2025 (SCI-Expanded, Scopus)
MicroRNAs and transcription factors are crucial components of the stress response network in plants. Arabidopsis callus tissues were exposed to drought and nitrogen deficiency conditions using the Murashige and Skoog culture medium. Sequencing identified 117 differentially expressed miRNAs within comparisons. mir156j, mir157b, mir166b, mir164a, mir160c, mir158b, mir156b, mir159b were among those detected. Upon combination stress, mir160c was upregulated (7.49) whilst downregulated (-6.87) in the drought-only treatment. Some of the known miRNAs identified included mir390, mir781, mir1543, mir829, mir8155, mir5643, and mir403. A total of 17,003 potential target genes were predicted for the identified Arabidopsis miRNAs. The target genes identified by psRNATarget were enriched in gene ontology categories and included processes such as Transcription regulator activity (GO:0140110), DNA binding transcription factor activity (GO:0003700), RNA helicase activity (GO:0003724), Gravitropism (GO:0048653), and Signal transduction (GO:0007165). Furthermore, among the transcription factor genes studied, HD-Zip protein family genes were detected to have varying expressions. UBC24 was upregulated by nitrogen deficiency, while REVOLUTA (controlled by mir165 and mir166) along with RH39 were downregulated after the nitrogen deficiency treatment. Our findings highlight varied expressions within miRNA families depending on the stress condition, along with strand-specific regulation and functions of miRNA target genes which can be leveraged to enhance agricultural productivity.