International Eurasian Conference on Biotechnology and Biochemistry (BioTechBioChem 2020), Ankara, Turkey, 16 December 2020, pp.716-724
An aptamer based electrochemical biosensor was designed for the detection of codeine. Polypeptidefunctionalized polymer (EBP) was coated on a glassy carbon electrode (GCE). Cysteamine modified gold
nanoparticles (AuNP-Cys) were attached on the polymer coated electrode surface via glutaraldehyde through
the amino functional groups of the polymer. Immobilization of aptamer was achieved via AuNP-Cys through
Au–SH affinity. Surface modifications were studied via electrochemical techniques such as cyclic
voltammetry, electrochemical impedance spectrometry, and differential pulse voltammetry. The designed
sensor showed an excellent electrocatalytic response towards the sensing of codeine with a wide linear range.