Polypeptide-functionalized Polymer Decorated with Gold Nanoparticles as Immobilization and Sensing Platform for Codeine Analysis

BULUT U., TİMUR S.

International Eurasian Conference on Biotechnology and Biochemistry (BioTechBioChem 2020), Ankara, Turkey, 16 December 2020, pp.716-724

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.716-724
  • Acibadem Mehmet Ali Aydinlar University Affiliated: Yes

Abstract

An aptamer based electrochemical biosensor was designed for the detection of codeine. Polypeptidefunctionalized polymer (EBP) was coated on a glassy carbon electrode (GCE). Cysteamine modified gold nanoparticles (AuNP-Cys) were attached on the polymer coated electrode surface via glutaraldehyde through the amino functional groups of the polymer. Immobilization of aptamer was achieved via AuNP-Cys through Au–SH affinity. Surface modifications were studied via electrochemical techniques such as cyclic voltammetry, electrochemical impedance spectrometry, and differential pulse voltammetry. The designed sensor showed an excellent electrocatalytic response towards the sensing of codeine with a wide linear range.