Pulsed-Field Gel Electrophoresis (PFGE) is a very important molecular research and diagnostic technique, used for the separation of very large DNA molecules. In this method the genome digested first by a restriction enzyme into large DNA fragments analyzed by PFGE. DNA fingerprinting is enabled the development of molecular epidemiology of infectious diseases. It is possible to identify the source and transmission routes of an epidemic by DNA fingerprinting of the strains of infectious agent isolated from a series of samples. Although DNA fingerprinting by PFGE is a very important and powerful technique, its application is very cumbersome, requires long time, experienced personnel and expensive equipment. These hinder the method from being widely used. In this project the aim is to develop an Observable Real Time PFGE system. Its efficiency is evaluated by comparing the genomic fingerprints of bacterial strains that were previously isolated from hospital infections by Observable and classical PFGE. Observable PFGE has the potential to eliminate the need for hazardous ultraviolet light and carcinogen ethidium bromide. It provided pictures at every stage of electrophoresis enabling to choose the best picture for analysis of the results. Since Observable PFGE enables to see the results early during electrophoresis we believe that compared to classical PFGE, it could save a lot of time and effort.