Akademik Veri Yönetim Sistemi
JOURNAL OF PERIODONTAL RESEARCH, cilt.58, sa.2, ss.256-261, 2023 (SCI-Expanded)
ObjectiveThis study aimed to evaluate the level of ADMA (asymmetric dimethylarginine), SDMA (symmetric dimethylarginine), and IL-1 beta (Interleukin-1 beta) in gingival crevicular fluid (GCF) from periodontitis patients and control subjects. BackgroundADMA and SDMA are potentially hazardous non-proteinogenic amino acids that limit nitric oxide (NO) synthesis and have many functions in various human disorders. ADMA causes a structural change in nitric oxide synthase, while SDMA blocks arginine cell uptake. Increased plasma ADMA has been widely recognized as a "trigger" initiating impaired NO bioavailability and vascular dysfunction, which ultimately leads to oxidative stress. MethodsTwenty-five patients with periodontitis (P) (Stage III, Grade C, n = 25) and 20 control (C) subjects were included in the study. The IL-1 beta level of GCF was measured by enzyme immunoassay (ELISA) and ADMA and SDMA by liquid chromatography-mass spectrometry (LC-MS/MS). ResultsPeriodontitis patients had higher clinical parameters than controls (p < .001). Levels of IL-1 beta, ADMA and SDMA GCF were statistically significantly higher in group P than in group C (respectively; p = .003, p < .0001, p < .0001). There was no difference in the ADMA/SDMA ratio (p = .312) between the groups. There were significant positive correlations between clinical periodontal parameters and IL-1 beta, ADMA, and SDMA levels (p < .05). ADMA and SDMA levels were significantly correlated with IL-1 beta (p < .05). ConclusionsThese findings suggest that ADMA and SDMA may be involved in the pathogenesis of the periodontal disease.