Introduction: The aim is to find out whether a hypothetical diagnostic benefit exists in the assessment of trace elements in the hair of bladder cancer patients by comparing the results with healthy. Materials and Method: In this prospective study, trace element analysis from patients with T1 bladder cancer (Group 1) and healthy (Group 2) was performed and their correlations were studied. Results: The trace elements determined in the order of higher levels were Sn, Ca, Cu, Zn and Mg in Group 1 and Zn, Ca, Mg, Cu and Sn in Group 2. When the levels of trace elements were compared between groups As, Bi, Ca, Co, Cs, Cr, Fe, Ga, Hg, K, Li, Rh, Sb, Sn and V levels were found to be statistically different (p<0.05). According to Pearson correlation, powerful correlations were found for B, Be, Ce, Co, Cs, Cu, Hg, K, Ni, Pb, Rb, Se, Sn, V and Zn element in group; for Cs, Cr, Ga, Hg, Mn, Pb, Rh, Sr and Zn in Group 2. Conclusion: This study demonstrated that heavy metal levels were higher in bladder cancer than in healthy. Our findings suggest that the heavy metals accumulated in the body may have an influence in bladder cancer development.