GABA and L-glutamic acid release in en bloc resection slices of human hippocampus: an in vitro microdialysis study

Goren M., Onat F., ÖZKARA Ç., Ozyurt E., Eskazan E., GÜLHAN R.

NEUROLOGICAL SCIENCES, vol.22, no.4, pp.297-302, 2001 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 4
  • Publication Date: 2001
  • Doi Number: 10.1007/s10072-001-8171-x
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.297-302
  • Keywords: in vitro microdialysis, mesial temporal lobe epilepsy, GABA (gamma-aminobutyric acid), L-glutamic acid, human hippocampus, EXTRACELLULAR GABA, AMINO-ACIDS, EPILEPSY, MODEL, RECEPTORS, THALAMUS, SEIZURE, NEURONS
  • Acibadem Mehmet Ali Aydinlar University Affiliated: No


The interaction of neurotransmitters has been a major interest in pathophysiological conditions like epilepsy. In vivo microdialysis has recently gained much validity in measuring neurotransmitter release in experimental animals. However, there is a paucity of data concerning its use in humans on the grounds of safety considerations. Microdialysis experiments were performed using die hippocampal head region removed from patients with medically intractable seizures, who underwent surgery for mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS). Following en bloc resection, the tissues were immediately transferred to the essential in vitro milieu. Slices were incubated in lactated Ringer's solution and microdialysis probes inserted into the slices were perfused with artificial cerebrospinal fluid (aCSF). When the K+ concentration of aCSF was elevated to 100 mM, GABA and L-glutamic acid levels increased by 293% and 177%, respectively. This method may serve as an experimental model for human brain, to throw more light on the interactions between GABA and L-glutamic acid in hippocampal tissues obtained from patients with MTLE-HS.