A Novel Biosensor Based on Glucose Oxidase for Activity Determination of α - Amylase
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY, cilt.39, sa.5, ss.298-303, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 39 Sayı: 5
- Basım Tarihi: 2011
- Doi Numarası: 10.3109/10731199.2011.574635
- Dergi Adı: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.298-303
- Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır
Özet
A glucose oxidase-based biosensor was developed for the determination of alpha-amylase activity. The determination method is based on monitoring the decrease in dissolved oxygen concentration related to the starch concentration, for which starch gives a reaction with alpha-amylase. Optimization parameters, including glucose oxidase amount, gelatin amount, and glutaraldehyde percentage for cross-linking, were investigated. The effects of pH, buffer system, and temperature on the biosensor system were also investigated. The biosensor had a linear relation to alpha-amylase activity and good measurement correlation between 0.66 and 9.83 U/ml. In sample analysis studies, alpha-amylase activity in baker's yeast was determined by the biosensor.