The determination of stage in nonmuscle urothelial carcinoma: Staining pattern of caspase-8

Sen Turk N., ESKICORAPCI S. Y., AYBEK Z., Tuncay L.

INDIAN JOURNAL OF PATHOLOGY AND MICROBIOLOGY, vol.61, no.2, pp.192-196, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 61 Issue: 2
  • Publication Date: 2018
  • Doi Number: 10.4103/ijpm.ijpm_161_17
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.192-196
  • Keywords: Caspase-8, murine double minute 2, p14(ARF), p53, urothelial carcinoma, CELL-CYCLE ARREST, BLADDER-CANCER, IMMUNOHISTOCHEMICAL ANALYSIS, EXPRESSION, APOPTOSIS, P14(ARF), P53, P21(WAF1/CIP1), PROGRESSION, SURVIVIN
  • Acibadem Mehmet Ali Aydinlar University Affiliated: Yes


Context: Urothelial carcinoma (UC) is one of the most frequent epithelial tumors worldwide. Aims: We aimed to investigate the protein expressions of caspase-8, p53, murine double minute 2 (mdm2), and p14(ARF) in nonmuscle UCs and to correlate the findings with clinicopathological characteristics. Settings and Design: A total of 50 patients who had pTa and pT1 tumors were analyzed. Subjects and Methods: The protein expressions of caspase-8, p53, mdm2, and p14ARF were analyzed by immunohistochemistry. Statistical Analysis Used: Chi-square test was done using SPSS version 16.0 (SPSS, Inc., Chicago, IL, USA). Results: Cytoplasmic caspase-8 expression was significantly higher in pT1 UCs while nuclear caspase-8 expression was significantly higher in pTa UCs (P = 0.005 and P = 0.011, respectively). Cytoplasmic caspase-8 expression was also higher in high-grade UCs (P = 0.035). The expression of p53, mdm2, and p14ARF was not also related with pathological stage or grade (P > 0.05 for all). The p14ARF expression was related with nuclear caspase-8 expression in most of the patients. Complete agreement among nonmuscle UCs for immunohistochemical expression of p14 and nuclear caspase-8 was seen in 41 cases, and the pairwise kappa agreement value was substantial (kappa = 0.614). The patients who had recurrence were positive for both p53 and mdm2 or either p53 or mdm2 (P = 0.025). Conclusions: These results suggested that the staining pattern of caspase-8 might be helpful for determining of the stages in nonmuscle UC. It was also showed that the expression status of p53 and mdm2 were related with the recurrence.