Synthetic peptide vaccine for Foot-and- Mouth Disease: synthesis, characterization and immunogenicity
TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.45, sa.6, ss.859-868, 2020 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 45 Sayı: 6
- Basım Tarihi: 2020
- Doi Numarası: 10.1515/tjb-2020-0110
- Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.859-868
- Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır
Özet
Background: The conjugations of antigenic synthetic peptide sequences with carrier polymers have opened new possibilities for the treatment of diseases. In this study, 135-161 peptide sequence of VP1 capsid protein of Foot-and-Mouth Disease was cross-linked with P(VP-co-AA) copolymer by covalent conjugation using water-soluble carbodiimide at different ratio of components (gamma=5, 7, 9, 11, 15) for the first time in the literature. Materials and methods: Bioconjugates were characterized by gel permeation chromatography and fluorescence spectroscopy to identify occurrences of the conjugates. After characterization, gamma=15 bioconjugate was determined as optimum conjugate for immunization studies and IC50 value is calculated as 1.227 mg/mL. By determining the nontoxic range, indirect ELISA were performed to evaluate the immune response elicited in balb/c mice by either peptide or P(VP-co-AA)-peptide bioconjugates (gamma=15). Two injections were applied to each group and high immune responses were obtained against gamma=15 conjugate compared to free peptide and control. Results and conclusion: At the end of 9-week, the general pattern of immunoreactivity was acquired as y=15>>peptide>control. Peptide formulated in the conjugated form had higher antibody response than free peptide and control (p<0.01, for all in both cases), this conjugate formulation put forward the adjuvant activity of P(VP-co-AA) polymer.