Akademik Veri Yönetim Sistemi
CENTRO 3R IV ANNUAL MEETING, The role of 3Rs in the age of One Health: where we are and where we’re going, Milan, İtalya, 13 - 15 Eylül 2023, cilt.1, ss.47
Ethical
Considerations in the Investigation of Ferulic Acid’s Therapeutic Effect on
Fertility in MSG-induced Testicular Damage
Anil Can (1), Merve Acikel Elmas (1),
Meltem Kolgazi (2), Serap Arbak (1), and Yesim Isil Ulman (3)
1Acibadem
University, School of Medicine, Department of Histology and Embryology
2Acibadem
University, School of Medicine, Department of Physiology
3Acibadem
University, School of Medicine, Department of History of Medicine and Ethics
This paper delas with ethical
aspects of a biomedical study comparing monosodium glutamate and ferulic acid over
male fertility. It benefits from the PREPARE Guidelines all through the research process.
Firstly, a thorough literature search was carried out to formulate an original
and robust study, and all referential sources were carefully listed in a
well documented manner to comply with research integrity, responsibility and
accountability while applying for the Ethics Committee. The
justification for the use of laboratory animals and suitability of the selected
species were based on scientific necessity as well as the ethical principle of Replace.
The research methodology was properly planned to prevent unnecessary use of animals.
Secondly, the correct number of animals in each group was carefully reckoned.
Calculation was carried out by using ANOVA power analysis with 80% power,
0.05 statistical significance and 0.6 effect size that determine to take eight
rats in each group as an optimum number to assess research hypothesis in line
with the ethical principle of Reduction. Thirdly, Refinement
measures were properly considered: the laboratory animals were scropulously
monitored regarding their physical and
psychological changes on daily basis; all precautions were taken to minimize pain
and distress on animals; discomfort were minimized by keeping rats in a
laboratory environment with a temperature of 22±2°C and a standard light/dark
(12/12 hours) cycle all through the study. Animals were randomly divided into 5
groups to minimize stress and to avoid social isolation. Pain was alleviated by
using anesthesia during euthanasia. In conclusion, this study aligns with research
integrity and the 3Rs principles by justifying the use of animals,
minimizing the number required, and implementing refinement measures to enhance
animal welfare in investigating the curative effect of ferulic acid on
fertility damage induced by MSG.
Citation: Anil Can, Merve Acikel Elmas, Melem Kolgazi, Serap
Arbak, Yesim Isil Ulman, “Ethical Considerations in the Investigation of
Ferulic Acid’s Therapeutic Effect on Fertility in MSG-induced Testicular Damage”,
CENTRO 3R IV ANNUAL MEETING, The role of 3Rs in the age of One
Health: where we are and where we’re going, Poster paper published in the
Book of Abstracts, Poster 22, Page 47, Università degli Studi di Milano-BicoccaSeptember
13-15, 2023