Assessment of the anti-quorum sensing effect of Lactobacillus sp. metabolites on expression levels of QS-related genes in Pseudomonas aeruginosa PAO1


KART D., RIZVANOĞLU S. S., ERYILMAZ M.

Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.77, sa.3, ss.311-318, 2020 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 77 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.5505/turkhijyen.2019.57873
  • Dergi Adı: Turk Hijyen ve Deneysel Biyoloji Dergisi
  • Derginin Tarandığı İndeksler: Scopus, Academic Search Premier, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.311-318
  • Anahtar Kelimeler: Anti-quorum sensing activity, Lactobacillus sp., Metabolites, RT-qPCR. pseudomonas aeruginosa
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

Objective: Pseudomonas aeruginosa is an important pathogen associated with nosocomial infections and its pathogenicity is mostly linked with the quorum sensing (QS) system. The aim of this study was to evaluate the anti-QS activity of the metabolites of vaginal Lactobacillus isolates and to investigate the effect of these metabolites on transcriptional regulation of QS related genes in P. aeruginosa PAO1. Methods: In this study, 13 Lactobacillus isolates that were previously identified by 16S rRNA gene sequence analysis were used. Metabolites of these isolates were assessed for the anti-QS activity by using Chromobacterium violaceum CV12472. The influence of metabolites on the expression of QS related genes was also examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results: All tested metabolites exhibited anti-QS activity with the appearance of a non-pigmented zone of C. violaceum. All tested quorum sensing-related genes (lasI, lasR, rhlR and mvfR) in P. aeruginosa PAO1 showed significant down-regulation after treating with the metabolites. Conclusion: New anti-infection agents from natural resources with a different mode of action are necessary due to the increasing occurrence of antibacterial resistance. Our study highlights the possible usage of Lactobacillus metabolites as an anti-QS agent against P. aeruginosa biofilm cells in vitro.