A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation


Mozioglu E. , Akgoz M., Tamerler C., KOCAGÖZ Z. T.

TURKISH JOURNAL OF BIOLOGY, cilt.38, ss.125-129, 2014 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 38 Konu: 1
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3906/biy-1308-7
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Sayfa Sayısı: ss.125-129

Özet

A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison.