Application of a new chemiluminescence method for the determination of glucose-6-phosphate dehydrogenase activity in healthy and enzyme-deficient individuals

GÜMÜŞLÜ S., Yucel G., Sarikcioglu S., SERTESER M.

Journal of Pharmacological and Toxicological Methods, vol.51, no.2, pp.169-174, 2005 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 2
  • Publication Date: 2005
  • Doi Number: 10.1016/j.vascn.2004.09.002
  • Journal Name: Journal of Pharmacological and Toxicological Methods
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.169-174
  • Keywords: Area under curve, Chemiluminescence, G-6-PD deficiency
  • Acibadem Mehmet Ali Aydinlar University Affiliated: No

Abstract

Introduction: A chemiluminescence (CL) technique, which determines the glucose-6-phosphate dehydrogenase (G-6-PD) activities in healthy, heterozygous, and completely enzyme-deficient individuals was applied. Methods: CL intensities were detected for 4 h at 15-min intervals in each sample with or without addition of G-6-PD substrates into the reaction mixture. Results: The results revealed an inverse correlation to the reference UV method (Zinkham method; r=-0.80). Furthermore, the CL assay was able to detect G-6-PD activities as low as 0.2 IU/gHb, which was not possible by the UV method. Discussion: In conclusion, we believe that this method offers a new diagnostic tool for the detection of G-6-PD activities in enzyme-deficient individuals and, because of its increased sensitivity, makes it amenable for determining the effects of different pharmaceutical agents on G-6-PD activity in tissue or cell cultures. © 2004 Elsevier Inc. All rights reserved.