Computational approaches for <i>de novo</i> design and redesign of metal-binding sites on proteins.


Akcapinar G. , Sezerman O. U.

Bioscience reports, cilt.37, 2017 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 37
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1042/bsr20160179
  • Dergi Adı: Bioscience reports

Özet

Metal ions play pivotal roles in protein structure, function and stability. The functional and structural diversity of proteins in nature expanded with the incorporation of metal ions or clusters in proteins. Approximately one-third of these proteins in the databases contain metal ions. Many biological and chemical processes in nature involve metal ion-binding proteins, aka metalloproteins. Many cellular reactions that underpin life require metalloproteins. Most of the remarkable, complex chemical transformations are catalysed by metalloenzymes. Realization of the importance of metal-binding sites in a variety of cellular events led to the advancement of various computational methods for their prediction and characterization. Furthermore, as structural and functional knowledgebase about metalloproteins is expanding with advances in computational and experimental fields, the focus of the research is now shifting towards de novo design and redesign of metalloproteins to extend nature's own diversity beyond its limits. In this review, we will focus on the computational toolbox for prediction of metal ion-binding sites, de novo metalloprotein design and redesign. We will also give examples of tailor-made artificial metalloproteins designed with the computational