Orexin peptides have role in the action mechanism of cholinergic anti-inflammatory pathway in the gastrointestinal tract

Kolgazi Gürcanlı M. , Çilingir S., Kılınç E. , Akyerli Boylu C.

Union of thrace universities 2nd international health sciences congress, Tekirdağ, Turkey, 15 - 17 November 2018

  • Publication Type: Conference Paper / Unpublished
  • City: Tekirdağ
  • Country: Turkey


Introduction:The cholinergic anti-inflammatory pathway is a neural mechanism, which has effects via vagus and α7 nicotinic acetylcholine receptors (α7nAchR). In the study the role of orexin on on the cholinergic anti-inflammatory pathway was investigated. Methods:Wistar albino rats (200-250gr) of both sexes were treated vehicle or orexin dual receptor antagonist (almorexan ALM; 30 mg/kg ip) for 3 days.  On 4th day, endotoxemia was induced with lipopolysaccharide (LPS-E. coli endotoxin serotype 0111:B4; 10 mg/kg ip). Half of the animals given LPS were administered vagal stimulation (VS) for 20 minutes. Six hours after LPS administration animals were decapitated. Collected liver, small intestine, colon samples were analyzed biochemically and mRNA expression of α7nAchR were measured. Proinflammatory cytokines, cortisol and some enzymes were measured in serum. ANOVA and Student’s t tests were used for statistical analysis. Results: Endotoxemia induced increase in TNF-α, IL-1β, IL-6, cortisol; AST, ALT and cortisol levels decreased with VS treatment and effects of VS were abolished by ALM. Increase in MPO and MDA levels because of sepsis decreased with VS treatment and the ALM diminished the VS effect in all tissues except liver. Depletion of GSH in the tissues was prevented by VS and these positive effects of VS were abolished by antagonist. mRNA expression of α7nAchR was increased with ALM significantly. Conclusion: Our findings reveal that orexin peptides have roles in the effects of cholinergic anti-inflammatory pathway on gastrointestinal tract. To determine the interaction between vagal fibers and peptide, new researches are needed.

This study was supported by TUBITAK (project no:2148185). 

Keywords: orexin,inflammation,vagus