Akademik Veri Yönetim Sistemi
Union of thrace universities 2nd international health sciences congress, Tekirdağ, Türkiye, 15 - 17 Kasım 2018
Introduction:The cholinergic
anti-inflammatory pathway is a neural mechanism, which has effects via
vagus and α7 nicotinic acetylcholine receptors (α7nAchR). In the study the role
of orexin on on the cholinergic anti-inflammatory pathway was investigated.
Methods:Wistar albino rats (200-250gr) of both sexes were treated vehicle or
orexin dual receptor antagonist (almorexan ALM; 30 mg/kg ip) for 3 days.
On 4th day, endotoxemia was induced with
lipopolysaccharide (LPS-E. coli endotoxin serotype 0111:B4; 10 mg/kg ip). Half
of the animals given LPS were administered vagal stimulation (VS) for 20
minutes. Six hours after LPS administration animals were decapitated.
Collected liver, small intestine, colon samples were analyzed
biochemically and mRNA expression of α7nAchR were measured. Proinflammatory
cytokines, cortisol and some enzymes were measured in
serum. ANOVA and Student’s t tests were used for statistical
analysis. Results: Endotoxemia induced increase in TNF-α, IL-1β, IL-6,
cortisol; AST, ALT and cortisol levels decreased with VS treatment and effects
of VS were abolished by ALM. Increase in MPO and MDA levels because of sepsis
decreased with VS treatment and the ALM diminished the VS effect in all tissues
except liver. Depletion of GSH in the tissues was prevented by VS and these
positive effects of VS were abolished by antagonist. mRNA expression of α7nAchR
was increased with ALM significantly. Conclusion: Our findings reveal that
orexin peptides have roles in the effects of cholinergic anti-inflammatory
pathway on gastrointestinal tract. To determine the interaction between vagal
fibers and peptide, new researches are needed.
This study was supported by
TUBITAK (project no:2148185).
Keywords: orexin,inflammation,vagus