The aim of the present study was to determine the long-term effects of different laparoscopic hemostatic techniques on ovarian reserve after ovarian cystectomy. Ninety patients with unilateral ovarian cysts were recruited and randomly distributed into 2 groups. Laparoscopic stripping cystectomy was performed in all patients. Afterward, cystectomy hemostasis was achieved via hemostatic suture or bipolar electrocoagulation. Serum levels of anti-Mullerian hormone (AMH) were determined preoperatively and postoperatively at 1, 3, and 12 months, and patients were evaluated for residual ovarian volume, antral follicle count, and pregnancy. The statistical difference was determined between the 2 groups in terms of AMH levels at 3 months (hemostatic suture group = 3.17 +/- 3.40 vs bipolar electrocoagulation group = 2.38 +/- 2.57, P = .006) and 12 months (hemostatic suture group = 3.71 +/- 3.09 vs bipolar electrocoagulation group = 2.78 +/- 2.85, P = .005). In addition, in the hemostatic suture group, there was no statistically significant difference between preoperative and postoperative AMH levels (P = .165) and between the postoperative antral follicle count (P = .779) and the residual ovarian volume (P = .248), whereas in the bipolar electrocoagulation group, postoperative AMH levels were lower than preoperative levels (P = .028) and postoperative residual ovarian volumes at 3 and 12 months were lower than those at 1 month (P = .001). Nonetheless, pregnancy rates were not significantly different (P = .546). Bipolar electrocoagulation is more destructive compared with hemostatic suture. However, the ovarian reserve does not decrease further during the follow-up period.