Akademik Veri Yönetim Sistemi
Uluslararası Biyokimya Kongresi, Antalya, Türkiye, 29 Ekim - 01 Kasım 2023, ss.80-81
Objective: Wheat contains about 8% to 15% of proteins divided
into the four so-called Osborne fractions according to their solubility:
albumins globulins gliadins and glutenins. The amylase/trypsin inhibitors
(ATIs) appear to be the major group of non-gluten fractions consisting of
albumins and globulins. ATIs are bifunctional proteins with theability to
inhibit both amylase and proteases activities, describing their role in
cereals.The alpha-amylase/trypsin
inhibitors (ATIs) are discussed as being responsible for non-celiac wheat
sensitivity (NCWS), besides being known as allergenic components for baker’s
asthma. Different approaches for characterization
and quantification including proteomics-based methods for wheat ATIs have been
documented. In these studies generally the major ATIs have been addressed wheats.
Aim: The main purpose of this study was to
determine alpha amylase trypsin inhibitors in Turkish wheat using an advanced
technique, HPLC-MS/MS.
Material and Method: 21
Turkish wheat samples were used. ATIs were measured by targeted tandem mass spectrometric analysis
(HPLC-MS/MS) after exraction.
Findings Thirteen (individual/common) biomarkers were established. Major ATIs (7–34%) were
differently represented in samples. In total, 11 individual biomarkers for
proteins P01083, P01083,P17314, P16850, P15851, P16159, P16159, P93602, P83207,
Q4U199, Q41540, Q41540 and two common biomarkers for proteins P01084/P01085 and P81496/Q43723/Q43691 were found.
Conclusion: Finally, to our knowledge, in the proposed study for the first time so far
the analysis of all Turkish wheat ATI entries reported.
Keywords: alpha-amylase/trypsin
inhibitors; wheats; peptides markers;
ass
spectrometry; LC-MRM-MS