Akademik Veri Yönetim Sistemi
JOURNAL OF EXPERIMENTAL MEDICINE, cilt.209, sa.12, ss.2263-2276, 2012 (SCI-Expanded)
The thymus generates T cells with diverse specificities and functions. To assess the contribution of cytokine receptors to the differentiation of T cell subsets in the thymus, we constructed conditional knockout mice in which IL-7R alpha or common cytokine receptor gamma chain (gamma(c)) genes were deleted in thymocytes just before positive selection. We found that gamma(c) expression was required to signal the differentiation of MHC class I (MHC-I)-specific thymocytes into CD8(+) cytotoxic lineage T cells and into invariant natural killer T cells but did not signal the differentiation of MHC class II (MHC-II)-specific thymocytes into CD4(+) T cells, even into regulatory Foxp3(+)CD4(+) T cells which require gamma(c) signals for survival. Importantly, IL-7 and IL-15 were identified as the cytokines responsible for CD8(+) cytotoxic T cell lineage specification in vivo. Additionally, we found that small numbers of aberrant CD8(+) T cells expressing Runx3d could arise without gamma(c) signaling, but these cells were developmentally arrested before expressing cytotoxic lineage genes. Thus, gamma(c)-transduced cytokine signals are required for cytotoxic lineage specification in the thymus and for inducing the differentiation of MHC-I-selected thymocytes into functionally mature T cells.