From in silico to in vitro: Modelling and production of Trichoderma reesei endoglucanase 1 and its mutant in Pichia pastoris

Akcapinar G., Gul O., Sezerman U. O.

JOURNAL OF BIOTECHNOLOGY, vol.159, no.1-2, pp.61-68, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 159 Issue: 1-2
  • Publication Date: 2012
  • Doi Number: 10.1016/j.jbiotec.2012.01.001
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.61-68
  • Keywords: Cellulase, Endoglucanase, Molecular modelling, Site directed mutagenesis, Trichoderma reesei, Pichia pastoris, AFFINITY ADSORPTION, CELLULASE, EXPRESSION, IMMOBILIZATION, ENZYMES, TRANSFORMATION, GLYCOSYLATION, PURIFICATION, STABILITY, CLONING
  • Acibadem Mehmet Ali Aydinlar University Affiliated: No


In this study, a major cellulase, namely endoglucanase 1 (EGI) from Trichoderma reesei was mutated by the introduction of four different lysine and glycine rich loops to create a hotspot for directed crosslinking of EGI away from the active site. The impact of the inserted loops on the stability of the enzyme was analyzed using molecular dynamics (MD) and the effect on the active site was studied using molecular mechanics (MM) simulations. The best loop mutation predicted in silico (EGI_L5) was introduced to EGI via site directed mutagenesis. The loop mutant EGI_L5 and EGI were both expressed in Pichia pastoris. Enzymes were characterized and their activities against soluble substrates such as CMC and 4-MUC were determined. Both enzymes exhibited similar pH and temperature activity and thermal stability profiles. Moreover, specific activity of EGI_L5 against 4-MUC was found to be the same as the native enzyme. (C) 2012 Elsevier B.V. All rights reserved.