One of problems after peripheral nerve injury and surgery is scar tissue formation. Although the continuity of axons is restored, often the axonal viability and recovery is blocked by scarring. For preventing from scar formation silicone tubes are widely used. In addition, in the present study was used hyaluronic acid, which prevents adhesion. The aim of the present study is to evaluate electrophysiological results on nerve regeneration obtained after incision and reconstruction of rat sciatic nerve using only silicone tubes and silicone tubes filled with hyaluronic acid. Electrophysiological measurements were performed at 30th and 60th days after surgery. The amplitude and latency of the compound muscle action potentials and nerve conduction velocities were calculated for each rat. Although sciatic nerve conduction velocities in all operated groups not reached control values, amplitude and latency values in group with silicone tube and in group with silicone tube filled with hyaluronic acid statistically achieved control values on day 60. Silicone tubes filled with hyaluronic acid had better results compared to the silicone tubes without hyaluronic acid. Hence, the use of silicone tubes and hyaluronic acid for the reconstruction of nerve injuries seems to give good results on axonal regeneration.