Label-free molecular detection of antibiotic susceptibility forMycobacterium smegmatisusing a low cost electrode format


DOĞAN GÜZEL F., Ghorbanpoor H., Dizaji A. N., AKÇAKOCA İ., Ozturk Y., Kocagoz T., ...More

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, vol.68, no.6, pp.1159-1166, 2021 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 68 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.1002/bab.2037
  • Journal Name: BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1159-1166
  • Keywords: antibiotic susceptibility, antimicrobial resistance, electrochemical biosensors, electrochemical impedance spectroscopy, square-wave voltammetry, 16S RIBOSOMAL-RNA, ELECTROCHEMICAL DETECTION, POINT, DNA, IDENTIFICATION, GENES
  • Acibadem Mehmet Ali Aydinlar University Affiliated: Yes

Abstract

Today, the emergence of antibiotic resistance in pathogenic bacteria is considered an important problem for society. Excessive consumption of antibiotics, long-term treatments, and inappropriate prescriptions continually increase the severity of the problem. Improving antibiotic stewardship requires improved diagnostic testing, and, therefore,in vitroantibiotic susceptibility testing is becoming increasingly important. This research details the development of an antibiotic susceptibility test forMycobacterium smegmatisusing streptomycin as antibiotics. This strain was selected because it is a member of the slow growingMycobacteriumgenus and serves as a useful surrogate organism forM. tuberculosis. A commercially available and low-cost screen-printed gold electrode in combination with a specifically developed nucleic acid probe sequence for the 16SrRNA region of the mycobacterial genome was employed to monitorM. smegmatisnucleic acid sequences using the techniques of square-wave voltammetry and electrochemical impedance spectroscopy. The results show that it was possible to detectM. smegmatissequences and distinguish antibiotic-treated cells from untreated cells with a label-free molecular detection. As a result, thein vitroantibiotic susceptibility test revealed thatM. smegmatisshowed sensitivity to streptomycin after a 24-H incubation, with the developed protocol representing a potential approach to determining antibiotic susceptibility more quickly and economically than current methods.