Akademik Veri Yönetim Sistemi
JOURNAL OF ARTHROPLASTY, cilt.37, sa.12, ss.2427-2430, 2022 (SCI-Expanded)
Background: The main objective of this study was to assess the sanitary measures of operating theaters using next-generation sequencing. Methods: Air was sampled from the operating room for the whole duration of 10 surgical days of "clean" (no infection cases) procedures (6 hip/knee arthroplasty and 4 spine cases). Controls consisted of sam-ples at the beginning of the day (1 hour before the surgery started) and at the end of the day after terminal cleaning. One additional control sample, consisting of a culture swab that was opened and exposed to the air for 5 seconds, was collected at each time point. All samples were sent for next -generation sequencing analysis (16S rRNA sequencing) for bacterial identification. Results: Overall, 306 samples were collected (159 controls and 147 experimental). Microbial DNA was detected in only 1 control sample, while 18 (12.2%) experimental samples were positive for microbial DNA. The most common organisms retrieved were Escherichia coli (6/18, 30%), Cutibacterium acnes (3/18, 15%), and Pseudomonas aeruginosa (2/18, 11.1%). There was no difference in positive samples between arthroplasty and spine cases (P > .05). Conclusion: Microbial organisms are not uncommonly present in the operating room air during hip and knee arthroplasties and spine procedures. (c) 2022 Elsevier Inc. All rights reserved.