ANTIBODY ARRAY-BASED IMMUNOSENSOR FOR DETECTING CARDIOVASCULAR DISEASE RISK MARKERS

Timucin C., Gul O., Kutuk O., Basaga H.

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, cilt.33, sa.3, ss.275-290, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1080/15321819.2011.638407
  • Dergi Adı: JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.275-290
  • Anahtar Kelimeler: ELISA, method comparison, multiplexed immunosensor, myoglobin, serum amyloid a, vascular cell adhesion molecule, PROTEIN, ELISA, MICROARRAYS, TECHNOLOGIES, CHALLENGES, VALIDATION, BIOMARKERS, CYTOKINES, ASSAYS
  • Acıbadem Mehmet Ali Aydınlar Üniversitesi Adresli: Hayır

Özet

Quantitative detection of proteins in multiplexed platforms presents technical advantages at clinical and laboratory settings compared to the monoplex ELISA method. With this purpose, we implemented a pilot study using in-house-designed sandwich-type antibody array for multiplexed detection of seven cardiovascular disease (CVD) risk markers and compared the performance of our immunosensor to conventional ELISA kits. Results indicated that our immunosensor can determine serum amyloid A (SAA), vascular cell adhesion molecule (VCAM), and myoglobin (MYO) concentrations accurately, precisely, and above all very much similar to ELISA. Hence, multiplexed detection and quantification of SAA, VCAM, and MYO with our immunosensor can be considered as a potential multiplexed alternative to the ELISA method.