ANTIBODY ARRAY-BASED IMMUNOSENSOR FOR DETECTING CARDIOVASCULAR DISEASE RISK MARKERS


Timucin C. , Gul O., Kutuk O., Basaga H.

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, vol.33, no.3, pp.275-290, 2012 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 3
  • Publication Date: 2012
  • Doi Number: 10.1080/15321819.2011.638407
  • Title of Journal : JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
  • Page Numbers: pp.275-290
  • Keywords: ELISA, method comparison, multiplexed immunosensor, myoglobin, serum amyloid a, vascular cell adhesion molecule, PROTEIN, ELISA, MICROARRAYS, TECHNOLOGIES, CHALLENGES, VALIDATION, BIOMARKERS, CYTOKINES, ASSAYS

Abstract

Quantitative detection of proteins in multiplexed platforms presents technical advantages at clinical and laboratory settings compared to the monoplex ELISA method. With this purpose, we implemented a pilot study using in-house-designed sandwich-type antibody array for multiplexed detection of seven cardiovascular disease (CVD) risk markers and compared the performance of our immunosensor to conventional ELISA kits. Results indicated that our immunosensor can determine serum amyloid A (SAA), vascular cell adhesion molecule (VCAM), and myoglobin (MYO) concentrations accurately, precisely, and above all very much similar to ELISA. Hence, multiplexed detection and quantification of SAA, VCAM, and MYO with our immunosensor can be considered as a potential multiplexed alternative to the ELISA method.