In Vitro Cultivation of Plasmodium berghei: Application of Drug Resistance Tests with Chloroquine and Artesunate


Ostan I., Kurt Ö. , Ozbilgin A.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.19, no.5, pp.909-912, 2013 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 5
  • Publication Date: 2013
  • Doi Number: 10.9775/kvfd.2012.8470
  • Title of Journal : KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Page Numbers: pp.909-912

Abstract

The aim of this study is to assess the drug resistance testing of Plasmodium berghei after short-term culture in vitro. First stage, the life cycle of the malaria parasites was completed in an average of 24 h inside the red blood cells in vitro. Second stage, the inhibition rates of Chloroquine and Artesunate on the infected erythrocytes were determined. The results showed that P. berghei parasites were completed their erythrocytic schizogony in short term in vitro cultivation. In the drug resistance tests with Chloroquine and Artesunate, antiplasmodial resistance did not occur with both drugs and the effect of Artesunate is higher than Chloroquine.