In this article, we report a 32-year-old female patient with isolated gluteal deep venous thrombosis, in whom immobility associated with sitting for long periods at work represented the major risk factor for her venous thrombosis. At admission, the patient had a week history of severe left hip pain which gradually increased in the last two days. Hip magnetic resonance imaging with contrast agent demonstrated left inferior gluteal vein thrombosis and perivascular inflammation which was subsequently supported by color Doppler ultrasonography. A search for possible causes of the thrombosis revealed no risk factor other than prolonged seated immobility at work. After anticoagulation with low molecular weight heparin and warfarin, her symptoms completely resolved in two weeks. In the absence of recognized risk factors, 21 century lifestyle associated immobility from prolonged sitting might be considered as the major contributing factor for venous thrombosis.