Effect of mineral trioxide aggregate cements on transforming growth factor beta 1 and bone morphogenetic protein production by human fibroblasts in vitro

Guven G., Cehreli Z. C., Ural A., SERDAR M. A., Basak F.

JOURNAL OF ENDODONTICS, vol.33, no.4, pp.447-450, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 4
  • Publication Date: 2007
  • Doi Number: 10.1016/j.joen.2006.12.020
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.447-450
  • Keywords: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide, bone morphogenetic proteins, enzyme-linked immunosorbent assay, mineral trioxide aggregate, transforming growth factor beta, END FILLING MATERIALS, TGF-BETA, CALCIUM HYDROXIDE, GENE-EXPRESSION, BIOCOMPATIBILITY, DIFFERENTIATION, GROWTH, TESTS, CELLS, MTA
  • Acibadem Mehmet Ali Aydinlar University Affiliated: No


The aim of this study was to evaluate and compare the effects of two commercial mineral trioxide aggregate (MTA) cements (ProRoot MTA and MTA Angelus) on transforming growth factor (TGF)-beta 1 and bone morphogenetic protein (BMP)-2 levels produced by cultured human gingival fibroblasts (HGFs). Human gingival tissues were obtained from individuals with healthy periodontium. HGFs were grown at 37 degrees C in humidified atmosphere of 5% CO2 in Dulbecco's modified Eagle's medium, supplemented with 10% fetal calf serum, penicillin, and streptomycin. After 24 and 72 hours of exposure to the MTA products, HGF viability was determined by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay. TGF-beta 1 and BMP-2 levels in cell-free culture media were determined by enzyme-linked immunosorbent assay. Cell viability of the test groups was significantly lower than that of control at 24 and 72 hours (p < 0.05) but showed an increase at 72 hours (p < 0.05). Both test groups showed increased TGF beta-1 levels at 72 hours (p < 0.05), whereas the MTA Angelus group displayed higher TGF beta-1 levels than control and ProRoot MTA groups at 24 and 72 hours (p < 0.05). At 24 hours, BMP-2 levels of the ProRoot group were significantly higher than that of MTA Angelus (p < 0.05). Both test materials increased the BMP-2 levels within time (p < 0.05) and displayed similar levels at 72 hours (p > 0.05). These results suggest that both MTA products are capable of stimulating HGF to produce BMP-2, whereas the stimulatory effect for TGF beta-1 is material dependent.